Hepatic PEPCK and G6Pase mRNA expressions were significantly higher (Supplementary Figure 2c, d), while GCK and FAS mRNA expression tended to be higher, in the simple steatosis, borderline-NASH and NASH groups than in the no steatosis group (Supplementary Figure 2e, f). Here, FAS is linked to metabolic dysfunction-associated steatohepatitis.