Consistent with the ELISA analysis results, the pro-IL-1β, IL-6 and TNF-α protein levels detected by western blot were significantly reduced in the shRNA vector + CGS group and the shRNA vector + SCH group compared with those in the control vector + CGS group and the control vector + SCH group at 8 h after the cells were exposed to low glucose and hypoxia (p < 0.05, Fig. 5A–C). Here, TNF is linked to spindle cell hemangioma.