To further explore the underlying molecular mechanism of G3BP1-mediated RCC EMT, we first searched for the oncogenic signaling pathways that can be affected by G3BP1 silencing, including STAT3, AP1, ISRE, TGFβ (p3TP), P53, NFAT, and WNT (TOPFlash)36 using pathway luciferase reporter system in RCC cells (Suppl Fig. 2). Here, TGFB1 is linked to renal cell adenocarcinoma.