To determine whether G3BP1 knockdown impacts RCC tumor growth and metastasis in vivo, we performed the orthotopic tumor xenografts with implantation of luciferase-labeled G3BP1 knockdown cells (ACHN-shG3BP1-luciferase) and control RCC cells (ACHN-Scr-luciferase) into the sub-renal capsule of adult nude mice left kidney. The gene discussed is G3BP1; the disease is renal cell adenocarcinoma.