In the first proof-of-concept application, we explored the use of the MiChip platform for multiplexed detection of three serum protein biomarkers: prostate-specific antigen (PSA), carcinoembryonic antigen (CEA), and α-fetoprotein (AFP), which are commonly used clinical biomarkers for prostate, colorectal, and hepatocellular cancers, respectively34,35. This evidence concerns the gene AFP and hepatocellular carcinoma.