CD45+CD33+ cells (leukemia blasts) in peripheral blood could be detected at the end stage of leukemia development (Additional file 1: Figure S1) and extensive infiltrations of AML cells could be detected in bone marrow, liver, and spleen confirmed by pathology (Fig. 4a) and flow cytometry (Fig. 4b). This evidence concerns the gene CD33 and acute myeloid leukemia.