As shown in Fig. 7, phosphorylations of PI3K (Tyr458/Tyr199), AKT (Ser473), and mTOR (Ser2448) decreased in the PCNP group and increased in the sh-PCNP group, suggesting that PCNP mediates the PI3K/AKT/mTOR signaling pathway in human neuroblastoma cells. The gene discussed is AKT1; the disease is neuroblastoma.