Although IHC interpretation was defined as positive if > 5% of tumor cells had nuclear staining for NKX2.2, CDX-2, PDX-1 and PTF1A, the majority of positive cases demonstrated diffuse nuclear immunoreactivity in both TMA and biopsy cases, and focal or patchy staining in rare cases were seen only in the gastric and pancreatic NETs. This evidence concerns the gene NKX2-2 and neoplasm.