In addition, the effect of miR-12528 on regulating the IGF-1R was validated in not only the A549 cells but also the eight human-derived cell lines; NCI-H226; -H596; -H460; SK-MES-1 (lung carcinoma); HEK293T (embryonic kidney); Hep G2 (liver hepatocellular carcinoma); MCF7 (breast carcinoma); and HeLa (cervix carcinoma) (Figure S1, Fig. 2c). This evidence concerns the gene IGF1R and breast carcinoma.