In agreement with data suggesting significant and strong co-expression of CX3CR1 with GPR65 and FFAR4 in specimens, western blot confirmed robust downregulation of GPR65 and FFAR4 in ovarian carcinoma cell lines transfected with CX3CR1-specific siRNAs (Fig. 7e, f). Here, CX3CR1 is linked to ovarian carcinoma.