To address if the LEC induced NOTCH3 increase was specific for WM852 melanoma cells, we co-cultured two additional melanoma cell lines derived from vertical growth phase primary tumor (WM793) and metastasis (WM165) (Tatti et al., 2011) with LECs and analysed the co-cultures for Notch3 expression by indirect immunofluorescence analysis (IFA). Here, NOTCH3 is linked to melanoma.