Remarkably, the androgen responsive LNCaP cells when treated with PEITC were able to differentiate into prostate cancer stem cells (PCSC) floating spheres, leading the researchers to hypothesize the epigenetic alterations exerted by ITC, decreased expression of DNMT1, increased histone H3K4 acetylation, and reactivated GSTP1 expression in the spheric PCSCs.65 Interestingly, PEITC treatment in LNCaP cells causes the epigenetic reactivation of Ras‐association domain family 1 isoform A (RASSF1A) gene by enhancing CpG demethylation at its promoter. This evidence concerns the gene GSTP1 and Familial prostate cancer.