PBMCs from 5 HCMV+ patients with a primary infection or a reactivation (KTR #104, #105, #107, #108, #109) were sorted and then amplified in vitro to reach a purity>95% (defined by tetramer staining using the HLA-E/UL40 complexes employed for sorting). The gene discussed is HLA-E; the disease is infection.