EEA1 and infection: Compared to phagosomes containing H37Rv or the complemented strain, which avoid EEA1 localization, phagosomes containing the secA2 mutant exhibited significantly higher EEA1 co-localization at both 1hr and 24hrs post infection (i. e. time following a 4hr period of initial uptake/infection) (Fig 1D and 1E, S1D Fig).