To confirm that PD-associated LRRK2 mutations increase S1292 phosphorylation,14 HEK293FT cells were transiently transfected with WT, S910A/S935A, S1292A, R1441C, Y1699C, K1906M, G2019S, I2020T, and G2385R LRRK2 constructs and blotted for total and phosphorylated LRRK2 antibodies (Fig. 1c). Here, LRRK2 is linked to Parkinson disease.