To determine if tumor growth impacts macrophage phenotypes, we constructed a NIH-3T3/Src (Y527F) cell line (NIH-3T3/Src) stably-expressing Src, a proto-oncogene tyrosine-protein kinase and used the conditioned medium of the cells to induce the polarization of Ana-1 cells, a mouse macrophage cell line. Here, SRC is linked to neoplasm.