RND1 and hepatocellular carcinoma: First, we measured the mRNA and protein expression of Rnd1 by real-time PCR and western blot in a normal liver cell line (L02) and in a series of HCC cell lines with varying metastatic potential (MHCC97H, HCCLM3, MHCC97L, HepG2, and Huh7)15.