Thus, we conclude that Rnd1 suppresses the activity of RhoA, leading to the inhibition of the Raf/MEK/ERK signaling pathway, and impairing the migration, invasion and EMT of HCC cells; in addition, Rnd1 silencing can be rescued by 5-Aza and SAHA in HCC cells (Fig. 7e). This evidence concerns the gene RAF1 and hepatocellular carcinoma.