Moreover, further flow cytometry analysis confirmed that a negative correlation was identified between Lnc-Tim3 expression and the percentage of IFN-γ+ CD8 T cells in the tumor-infiltrating CD8 T cells of HCC patients (Fig. 1d), suggesting that the presence of Lnc-Tim3 is associated with the exhaustion of CD8 T lymphocytes. This evidence concerns the gene CD8A and hepatocellular carcinoma.