Among the main findings of this research: (1) The HPLC-MS/MS-based approach described here proved a sensitive and specific methodology for measuring the steroids under study; (2) The use of an IS prevented interferences in extraction caused by differences in matrix complexities between HIV-TB and HD; (3) Notably, HIV-TB patients exhibited higher plasma levels of DHEA, AET and 7-oxo-DHEA compared with HD; 4) 7-oxo-DHEA levels were positively related with absolute CD4+ T cell counts, CD4+ T cell nadir values and the development of pulmonar instead of extrapulmonar TB. This evidence concerns the gene CD4 and tuberculosis.