In addition, acute liver injury by CCl4 will presumably be attenuated by mild pre-existing liver diseases such as nonalcoholic fatty liver disease (NAFLD) or alcoholic fatty liver (AFL) due to increased CCl4 metabolism via CYP2E1, the isoenzyme commonly found with increased contents and enzymatic activities in both, NAFLD and AFL [69]. This evidence concerns the gene CYP2E1 and metabolic dysfunction-associated steatotic liver disease.