To confirm that CD2AP binding to CHIKV and SFV nsP3 HVD is relevant in context of virus replication, human osteosarcoma cells (HOS) were infected with wt CHIKV, CHIKVmutM2, wt SFV or SFVmutM2 at MOI 10. This evidence concerns the gene SH2D3C and osteosarcoma.