To validate the effects of JIB-04 on the mRNA expression of β-catenin target genes, we measured the mRNA levels of CSC-associated genes (CD44, LGR5, DKK1, ALDH1A3, ALDH1B1, CD24, and SOX4) by qRT-PCR in three different colorectal cancer cell lines after treament with JIB-04. Here, CD44 is linked to colorectal cancer.