Next, to verify the effects of YAP1 on NSCLC tumorigenesis and the EMT program in an in vivo model, we generated a luciferase-labeled stable YAP1 knockdown human lung cancer A549 cell line (Luc-shRNA-hYAP1-NEO) and a scrambled shRNA human lung cancer A549 cell line (Luc-shRNA-Scramble-NEO). Here, YAP1 is linked to non-small cell lung carcinoma.