A drop of Sirt1 expression was found in DN and knockdown of Sirt1 expression could abolish the beneficial effect of an active component against renal damage in DN3–6, while activating Sirt1 reversed the inflammation and fibrosis induced by high glucose in rat glomerular mesangial cells (MCs) and silencing Sirt1 could promote fibrosis factors and inflammation factors in high glucose-cultured rat glomerular measangial cells through promoting its downstream molecule hypoxia-inducible factor-1α (HIF-1α) expression7. Here, HIF1A is linked to liver dysplastic nodule.