As Akt is a downstream target of neuronal insulin receptor activation that regulates surface DAT expression, and, therefore, DA tone, we hypothesized that blunted striatal insulin receptor signaling after 2-weeks on HF diet, as shown by parallel decreases in Akt signaling, would decrease AMPH-evoked neural responses, consistent with decreased surface DAT expression. This evidence concerns the gene AMPH and hydrops fetalis.