HF feeding also decreased activity of the insulin-responsive kinase Akt (Fig 1A), a mechanism linked to reductions in striatal DAT surface expression and activity previously demonstrated in a 4-week HF DIO model [8], suggesting that a similar mechanism may contribute to the dysregulated AMPH-evoked brain activation seen in HF-fed animals here. This evidence concerns the gene SLC6A3 and hydrops fetalis.