Functional analysis of approximately 50 cancer-associated mutations in eight different PKC family members, including PKCβ, indicated that most of the mutations are loss-of-function, reducing or eliminating kinase activity24; evolutionary analysis performed by KinView25, a sequence visualization tool embedded in ProKinO, predicted and experimentally validated a PKCβ loss-of-function mutation D523N in the F-helix, although it is not a mutation-PTM overlapping site (Fig. 4). Here, PRRT2 is linked to cancer.