While treatment with IL PV-10 alone or in combination with anti-PD-1 antibody led to a delay in growth of treated tumor, only the combination therapy was able to delay the growth of uninjected bystander tumor (Fig 5, p<0.05 compared to PBS and PV-10, p = 0.06 compared to anti-PD-1 alone). The gene discussed is PDCD1; the disease is neoplasm.