Endogenous IDH1 mutant glioma cells are difficult to grow and maintain in culture—indeed, we lost one line during the process of these experiments—and thus, the first experiments to determine the effect of the IDH1 mutation on cells involved exogenous overexpression of the IDH1 mutant gene in an IDH WT background and performing mass spectroscopy to find differences in the levels of the various metabolites [13, 19, 38–40]. Here, IDH1 is linked to glioma.