Next we investigated the effect of MEG3 and miR-let-7c-5p co-processing on NLRC5, AML-12 cells was co-transfected with MEG3 siRNA and miR-let-7c-5p inhibitor, then the protein level of NLRC5 was determined by western blot. The gene discussed is MEG3; the disease is acute myeloid leukemia.