BHLHE41 and leukemia: To elucidate the role of SHARP1 in human MLL-AF6 leukemia, we performed knockdown experiments using two independent lentiviral shRNAs against SHARP1 (shSHARP1 #1 and #2) and a shRNA against GFP (shGFP) as a control in ML-2, CTS and SHI-1 cells.