To further investigate the role of SHARP1 in the development of MLL-AF6 AML, we transduced fluorescence-activated cell sorting (FACS)-sorted lineage (Lin)- Sca1+ c-kit+ (LSK) cells from bone marrow (BM) cells of Sharp1+/+ and Sharp1−/− mice23 with the MLL-AF6 fusion gene as described previously12. The gene discussed is BHLHE41; the disease is acute myeloid leukemia.