To confirm these findings in another MLL-AF6 AML cell line, we performed an independent ChIP-seq analysis of SHI-1 cells which expresses both MLL and MLL-AF6, demonstrating that MLLN binds to SHARP1 gene loci, as well as posterior HOXA genes locus (Fig. 2c). Here, BHLHE41 is linked to acute myeloid leukemia.