PHF1 and Alzheimer disease: Western blot analysis showed a significant increase of total (TG5 and 17025 antibodies) and phosphorylated tau at Ser202 (CP13), Thr231 (AT180), Ser396/404 (PHF1) residues in hippocampal lysates of vehicle-treated 3xTg-AD mice compared with WT groups (P < 0.05; Fig. 4B).