Overall, these data indicate that the most prevalent disruptions in rhythmic DNA methylation in Snord116+/− cortex are ZT6 nadirs lost and ZT16 nadirs gained in PWS, corresponding with genes showing increased expression at ZT6 (p = 3.54 × 10−74 and p = 5.49 × 10−83, respectively) and transcription factor binding sites in promoters and enhancers. This evidence concerns the gene SNORD116 and Prader-Willi syndrome.