A panel of eight pro- and anti-inflammatory cytokines and chemokines representing the M1 type of response (CXCL10, IL-23, IL-1 β, TNFα, IL-6 and MMP-9) and the M2 type of response (CCL22, IL-10) was selected to evaluate the effect of CRC cell line derived EVs on the monocyte and macrophage secretory profiles. Here, MMP9 is linked to colorectal carcinoma.