In this work, we investigate the effect of PEG-PPO-PEG hydrogels on alpha-synuclein aggregation using an in vitro cellular model that was previously developed [14], where human neuroblastoma cells are infected with alpha-synuclein filamentous aggregates produced by protein misfolding cyclic amplification (PMCA) [14]. The gene discussed is SNCA; the disease is neuroblastoma.