TNF and alkaline phosphatase measurement: Given to animals subjected to experimental AP, this conjugated compound (HPDM-rhein) reduced the severity of AP (lower amylase, milder injury to the acinar cells in terms of vacuolization and destruction), the neutrophilic inflammatory infiltrate (indicated by MPO and histological data), the local and systemic inflammatory reaction (lower levels of IL-6 and TNF-α in plasma, pancreas, and lung), the lung histological damage (alveolar wall thickening by edema and cellular infiltration), and the volume of ascites.