After 2 h infection of RAW 264.7 macrophages with SP and two strains of PA (PA01, PA14) at three different MOI (1, 5, and 10) there was noticeable decrease in both cell-associated Wnt5A (Figure 2A: ~14−80% decrease, Figure 2B: ~20–64% decrease, Figure 2C: ~27–50%) and secreted Wnt5A (Figure 2D: ~34–50% decrease). Here, WNT5A is linked to infection.