GUCY1B1 and intrahepatic cholangiocarcinoma: We examined the expression profile of NO targets in FACS sorted ICC from enzymatic dispersions of small intestinal muscles, as previously described (Baker et al., 2016), and characterized expression of guanylate cyclase 1 soluble subunits alpha 1 and beta 3: Gucy1a1 and Gucy1b1, protein kinase cGMP-dependent type 1: Prkg1, and inositol-1,4,5 triphosphate receptor I-associated G kinase substrate (IRAG) (aka murine retrovirus integration site 1 homolog): Mrvi1 transcripts.