Similar to the result of Th17 and Treg cell percentages, DAPT treatment resulted in reduced RORγt mRNA expression in cultured CD4+ T cells of PV patients (F = 176.052, P < 0.01, Figure 5(d)), while there was no significant change in Foxp3 mRNA expression (F = 1.025, P > 0.05, Figure 5(e)). Here, FOXP3 is linked to acquired polycythemia vera.