CD4+ T cell growth of PV patients was determined by the CCK-8 assay after DAPT or DMSO treatment for 72 hours, and ANOVA revealed that there was no significant difference in the optical density values at 450 nm among DAPT-treated groups and control groups (F = 2.343, P > 0.05, Figure 6). Here, CD4 is linked to acquired polycythemia vera.