Some indication about the concentration of circulating hypermethylated RASSF1A in serum from breast cancer patients, between 1 and 200 ng mL−1, is given by Kristiansen et al. 42, while the best cut-off for circulating serum RASSF1A to differentiate the hepatocellular carcinoma is 13 pM according to Mansour et al. 51. The gene discussed is RASSF1; the disease is breast cancer.