In lean-V rat adipocytes cultures, NASH-V-CM and TNFα induce the release of aforementioned cytokines and up-regulated the TNFR1/NFκB expressions in cell lysates, which were dose-dependently abolished by vitamin D co-incubation (Fig 4C and 4D). The gene discussed is TNFRSF1A; the disease is metabolic dysfunction-associated steatohepatitis.