CXCR4 and breast carcinoma: To confirm CXCR4 expression, first we screened for CXCR4 expression in our breast carcinoma cells lines MDA-MB-468, MDA-MB-231, MCF-7, DU4475, as well as in THP-1, HUVEC, U937, and Jurkat through RT-PCR analysis (Figure 5) Total RNA was prepared and subjected to RT-PCR analysis to detect CXCR4-specific mRNA.