However increased HA synthesis and signaling is vital for anchorage dependent monolayer growth of NSCLC cell lines with AGL loss, since loss of HAS2, RHAMM or treatment with HA synthesis inhibitor 4MU have a major inhibitory effect on the anchorage dependent monolayer growth of AGL knockdown NSCLC cells but not control cells. This evidence concerns the gene HAS2 and non-small cell lung carcinoma.