Through metabolomics and transcriptomic analysis we identified that loss of AGL makes bladder cancer cells dependent on Serine Hydroxymethyltransferase 2 (SHMT2) driven glycine synthesis and Hyaluronic Acid (HA) Synthase 2 (HAS2) driven HA synthesis for aggressive growth [3–5]. This evidence concerns the gene SHMT2 and urinary bladder carcinoma.