However increased HA synthesis and signaling is vital for anchorage dependent monolayer growth of NSCLC cell lines with AGL loss, since loss of HAS2, RHAMM or treatment with HA synthesis inhibitor 4MU have a major inhibitory effect on the anchorage dependent monolayer growth of AGL knockdown NSCLC cells but not control cells. Here, AGL is linked to non-small cell lung carcinoma.