In this study, we aimed to analyze DFNA5 methylation and expression in the largest breast adenocarcinoma patient cohort to date (Fig. 1) using publicly available data from The Cancer Genome Atlas (TCGA) in order to further unravel the role of DFNA5 as detection and/or prognostic marker in breast cancer [37]. This evidence concerns the gene GSDME and breast adenocarcinoma.