Moreover, we also performed qRT-PCR to measure the expression of ZFAS1 in human bladder cancer cell lines (T24, RT4, J82, and SW780) and human normal uroepithelium cell line (SV-HUC-1), and observed that bladder cancer cell lines suggested higher expression levels of ZFAS1 compared with normal uroepithelium cell line (P<0.001, Figure 1C). Here, ZFAS1 is linked to urinary bladder carcinoma.