More importantly, our current data indicated that although swimming alone may slightly, or metformin may moderately inhibit the GSK3β activity via increasing its phosphorylation level, only did the combined intervention elicit most effective inhibition of GSK3β activity or promotion of GS activity, thereby alleviating the hyperglycemia in GDM mice through boosting the energy storage or glucose conversion to glycogen in skeletal muscle. The gene discussed is GSK3B; the disease is gestational diabetes.