In the present study, we found that swimming or metformin alone may significantly ameliorate the SREBP2 protein levels in livers and skeletal muscle tissues from GDM mice, and that only combined therapy of swimming plus metformin totally suppressed SREBP levels in both organs compared to the NC group, suggesting that the combined intervention may be the most effective therapy in correcting the dysregulation of cholesterol synthesis both in liver and skeletal muscle from GDM mice. This evidence concerns the gene SREBF2 and gestational diabetes.