NOS2 and infection: RAW 264.7 cells were transiently transfected with either the iNOS promoter reporter construct pTK-3XNS or the NF-κB consensus luciferase reporter construct (p6κB-Luc) prior to infection with either L. infantum WT, Δlpg1 or Δlpg1+LPG1, or stimulation with LPS.