Ginsenoside Rb1 reduced the anxiety index, increased the risk assessment, reduced grooming behaviors in the EPM test, and increased the total number of line crossings of an open field in single prolonged stress (SPS)-induced model and in model rats of post-traumatic stress disorder (PTSD) by increasing the expression of neuropeptide Y induced by SPS-induced decrease in hypothalamic; increase the expression of tyrosine hydroxylase in locus coeruleus and decrease the mRNA expression of BDNF in hippocampus. Here, BDNF is linked to post-traumatic stress disorder.