When AFP levels were <20 ng/ml, the triplex miRNA panel still sustained its diagnostic accuracy in distinguishing HCC from the other groups (CHB patients AUC = 0.922, LC patients AUC = 0.744, patients with either CHB and LC (CHB+LC) AUC = 0.836, non-HCC individuals (CHB+LC+HC) AUC = 0.862). The gene discussed is AFP; the disease is hepatocellular carcinoma.