In contrast, LIMD1 and CDC25A showed high nuclear expressions in both basal/parabasal (80.06%, 82.76% respectively) and spinous layers (80.06%, 74.71%) of normal epithelium with significant loss of their expression during development of dysplasia (63.88%, 52.78%) and HNSCC (43.42%, 47.37%) (Fig 3A and 3B). Here, LIMD1 is linked to head and neck squamous cell carcinoma.