After normalization for cell number, no GPC-1 was detected for healthy control UCS, but a band representing cell surface expressed GPC-1 (~70kDa; equivalent to that seen for GPC-1 positive DU-145 cell extracts; a slight difference in size between UCS and DU-145 extract attributed to different heparinase treatment protocols is observed) was detected for prostate cancer (Fig 2). This evidence concerns the gene GPC1 and Familial prostate cancer.