Among the various B-cell subsets, naive mature B-cells are unresponsive to the TLR9 differentiation pathway, while transitional B-cells, IgM MBCs, and MZB-cells respond to TLR9 stimulation and differentiate into ASPC predominantly secreting IgM but also some IgG and IgA, similar to CLL B-cells. Here, TLR9 is linked to B-cell chronic lymphocytic leukemia.